3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3

I-CHAPS (3--[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) isihlanzi esivame ukusetshenziswa ku-biochemistry kanye ne-molecular biology.Isihlanzi se-zwitterionic, okusho ukuthi sinokubili iqembu elikhokhiswe kahle nelibi.

I-CHAPS yaziwa ngekhono layo lokunyibilikisa kanye nokusimamisa amaprotheni olwelwesi lwama-membrane, okulwenza lusebenziseke ezinhlotsheni ezihlukahlukene ezifana nokukhipha amaprotheni, ukuhlanzwa, kanye nokuhlukaniswa kwezinhlamvu.Iphazamisa ukusebenzisana kwe-lipid-protein, ivumela amaprotheni e-membrane ukuthi akhishwe esimweni sawo somdabu.

Ngokungafani nezinye izihlanzi, i-CHAPS imnene uma kuqhathaniswa futhi ayiwakhiqizi amaphrotheni amaningi, okwenza kube ukukhetha okuhle kakhulu ekugcineni ukwakheka nokusebenza kwamaprotheni ngesikhathi sokuhlolwa.Kungasiza futhi ukuvimbela ukuhlangana kwamaprotheni.

I-CHAPS ivame ukusetshenziswa kumasu afana ne-SDS-PAGE (i-sodium dodecyl sulfate polyacrylamide gel electrophoresis), i-isoelectric focusing, kanye ne-Western blotting.Ibuye isetshenziswe kaningi ezifundweni ezibandakanya ama-enzyme aboshwe ulwelwesi, ukudluliselwa kwesignali, nokusebenzisana kwamaprotheni-lipid.

Oda noma Cela I-Quote

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isicelo kanye Nomphumela

I-Protein Extraction: I-CHAPS ijwayele ukusetshenziselwa ukukhipha amaprotheni e-membrane kumasampula ebhayoloji.Kuyasiza ekuncibilikiseni lawa maprotheni futhi agcine ukwakheka kwawo komdabu.

Ukuhlanzwa Kwamaprotheni: I-CHAPS isetshenziswa kumasu ahlukahlukene okuhlanza amaprotheni, njenge-affinity chromatography.Ingangezwa kuma-buffers wokuhlanza ukuze i-solubilize futhi iqinise amaprotheni e-membrane ngesikhathi senqubo yokuhlanza.

I-Protein Characterization: I-CHAPS ivame ukusetshenziswa ocwaningweni olubandakanya ukuhlukaniswa kwamaphrotheni olwelwesi.Isiza ukugcina ukwakheka nokusebenza kwamaprotheni phakathi nezinqubo zokuhlola ezifana nokuhlolwa komsebenzi we-enzyme, ukusebenzisana kwamaprotheni namaprotheni, nokuhlaziya okubonakalayo.

Izifundo Ze-Membrane Protein: Amaprotheni e-Membrane adlala indima ebalulekile ezinqubweni eziningi zamaselula.I-CHAPS ivame ukusetshenziswa ocwaningweni oluhlobene nokudluliswa kwesignali, ukusebenza kwesiteshi se-ion, ukusebenzisana kwamaprotheni-lipid, kanye ne-membrane protein crystallization.

I-Electrophoresis: I-CHAPS isetshenziswa kumasu afana ne-SDS-PAGE kanye ne-isoelectric egxile ekunyibilikiseni amaprotheni olwelwesi lwama-membrane nokwenza kube lula ukuhlukana nokuhlaziya.

Ukupakishwa komkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	I-C32H58N2O7S
Isivivinyo	99%
Ukubukeka	I-crystalline powder emhlophe
Inombolo ye-CAS.	75621-03-3
Ukupakisha	Ezincane nezinkulu
I-Shelf Life	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	ISO.