1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride CAS:25952-53-8

I-1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, eyaziwa nangokuthi i-EDC hydrochloride noma i-EDAC, iyinhlanganisela yamakhemikhali evame ukusetshenziswa ku-biochemistry kanye ne-molecular biology njenge-ejenti yokuhlanganisa ukuze kusebenze amaqembu e-carboxyl. Iyimpuphu emhlophe ekristalu encibilika kakhulu emanzini.

I-EDC hydrochloride isetshenziswa kakhulu ekwakhiweni kwama-peptide namaprotheni ngokwenza kube lula ukwakheka kwama-amide bond phakathi kwamaqembu e-carboxyl nama-amino. Isebenza ngokwenza amaqembu e-carboxyl asebenze, asabela namaqembu ama-amino lapho kukhona i-coupling agent efana ne-N-hydroxysuccinimide (NHS). Lokhu kusabela kuphumela ekwakhekeni kwama-amide bond azinzile.

I-EDC hydrochloride isetshenziswa futhi ocwaningweni lwe-DNA ne-RNA lokuguqula nokuxhumanisa ama-nucleic acid, kanye nokuvimbela ukunyakaza kwama-enzyme namanye ama-biomolecule ezindaweni. Kuyi-reagent eguquguqukayo engathuthukisa ukusebenza kahle kanye nokucaciswa kwalezi zindlela zokusabela.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukwenziwa kwe-Peptide kanye namaprotheni: I-EDC hydrochloride ivame ukusetshenziswa njenge-ejenti yokuhlanganisa ekwakhiweni kwe-peptide kanye namaprotheni. Ikhuthaza ukwakheka kwezibopho ze-peptide phakathi kweqembu lama-amino le-amino acid eyodwa kanye neqembu le-carboxyl lenye i-amino acid, okwenza kube lula ukwenziwa kwama-peptide namaprotheni ngokwezifiso.

Ukuguqulwa kwe-Nucleic acid: I-EDC hydrochloride isetshenziswa ocwaningweni lwe-nucleic acid ukuguqula nokuxhumanisa i-DNA ne-RNA. Ingenza kube lula ukuhlanganiswa kwama-nucleic acid namanye ama-molecule, njengedayi e-fluorescent noma ama-biomolecule, okuvumela izinhlelo zokusebenza ezahlukahlukene ezingezansi njengokulebula, ukuhlanganisa, kanye nokutholwa.

Ukumiswa kwe-enzyme: I-EDC hydrochloride isetshenziswa ezinqubweni zokumiswa kwe-enzyme, lapho ama-enzyme enamathiselwa khona ezindaweni noma kuma-matrices ukuze kuthuthukiswe ukuzinza kwawo kanye nokusetshenziswa kwawo kabusha. I-EDC hydrochloride ingavuselela amaqembu e-carboxyl ebusweni futhi ikhuthaze ukubopha kwe-covalent kwama-enzyme ezintweni.

Ukuhlanganiswa kwe-Bio: I-EDC hydrochloride isetshenziswa ekuhlanganisweni kwe-biomolecules namanye ama-molecule noma izindawo. Ingavuselela amaqembu e-carboxyl kokubili kuma-biomolecules nakwamanye ama-molecule athakazelisayo, okwenza kube lula ukuhlanganiswa kwawo futhi kwakheke ukuxhumana okuzinzile.

Izinhlelo zokulethwa kwemithi: I-EDC hydrochloride isetshenziswa ekulungiseleleni izinhlelo zokulethwa kwemithi, lapho isiza khona ekuhlanganisweni kwemithi noma ama-molecule okwelapha nezinto zokuthwala, njengezinhlayiya ezincane noma ama-polymer. Lokhu kuhlanganiswa kungathuthukisa ukuzinza kwemithi, ukulethwa okuqondiwe, kanye nokukhululwa okulawulwayo.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C8H18ClN3
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe ekristalu
Inombolo ye-CAS	25952-53-8
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.