I-Uridine,2′-deoxy-5-ethynyl- CAS:61135-33-9

I-5-ethynyl-2′-deoxyuridine (EdU) iyi-analog ye-nucleoside evame ukusetshenziswa emkhakheni we-molecular biology kanye nocwaningo lwe-cell biology. Ivame ukusetshenziswa ukulebula nokuthola i-DNA esanda kuhlanganiswa kumaseli aphilayo ngenqubo eyaziwa ngokuthi "i-click chemistry." Le ndlela ivumela ukubona nokulinganisa ukuphindaphindwa kwe-DNA kanye nokwanda kwamaseli. I-EdU isibe yindlela ethandwayo esikhundleni se-thymidine analog bromodeoxyuridine (BrdU) yendabuko ngenxa yokuhambisana kwayo ne-fluorescence microscopy kanye nokungabikho kwezinyathelo ezinzima zokuguqulwa kwe-DNA ngesikhathi sokutholwa. Ngaphezu kwalokho, isakhiwo samakhemikhali se-EdU sivumela ukutholwa okuqondile nokusebenza kahle, okwenza kube ithuluzi eliwusizo lokufunda ukwakheka kwe-DNA kanye nokuguquguquka komjikelezo weseli.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukuhlolwa Kokwanda Kwamaseli: I-EdU ingafakwa ekuphindaphindeni i-DNA ngesikhathi sesigaba se-S somjikelezo weseli. Lokhu kuyenza ithuluzi eliwusizo lokufunda ukwanda kwamaseli kanye nokwakheka kwe-DNA.

I-Click Chemistry: I-EdU ivame ukusetshenziswa kanye ne-click chemistry, ukusabela okukhethayo nokunenzuzo ephezulu okusetshenziselwa ukuthola ama-biomolecule. Le ndlela ivumela ukutholwa okuqondile kwe-DNA enelebula le-EdU kumaseli nezicubu.

I-Fluorescence Microscopy: Ukufakwa kwe-EdU kuvumela ukubonakala kokwenziwa kwe-DNA ngaphansi kwe-fluorescence microscope. Le ndlela inikeza ithuluzi eliwusizo lokufunda ukuhlukana kwamaseli nokwanda kwawo.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C11H12N2O5
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	61135-33-9
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.