I-Trypan Blue CAS: 72-57-1 Intengo Yomkhiqizi

Ukuhlolwa Kokuphila Kwamaseli: I-Trypan Blue ivame ukusetshenziswa ekuhlolweni kokuphila kwamaseli ukuze kutholakale inani lamaseli asebenzayo kusampula ethile. Amaseli asebenzayo awafaki udayi futhi abonakala ecacile, kuyilapho amaseli angasebenzi ethatha udayi, ewenza abe luhlaza okwesibhakabhaka. Ngokufaka imibala kumaseli afile, abacwaningi bangahlola iphesenti lamaseli asebenzayo futhi banqume ukuthi amangqamuzana asebenza kahle noma ukuthi ayingozi kangakanani.

Ukubalwa Kwamaseli: I-Trypan Blue ingasetshenziswa ekubalweni kwamaseli okunembile ngokuhlukanisa aphilayo namaseli afile. Ngokufaka amabala kumaseli angaphili, abacwaningi bangawakhipha ekubalweni kwamaseli, okuhlinzeka ngemiphumela enembile nethembekile.

Ukuhlonza Amaseli Afile: I-Trypan Blue isiza ekuboneni amaseli afile noma inqwaba yamaseli angaphili ezitshalweni noma kumasampula ezicubu. Abacwaningi bangahlukanisa kalula amaseli aphilile nafile ngaphansi kwe-microscope ngokudaya okuluhlaza okwesibhakabhaka kwamaseli angaphili.

Ukuhlela Amaseli/Ukuhlanzwa Kwamaseli: Ukudaya okuluhlaza okwesibhakabhaka kwe-Trypan ngezinye izikhathi kusetshenziswa njengesinyathelo sokuqala sokuhlunga amaseli noma izindlela zokuhlanza amaseli. Ngokudaya amaseli afile, abacwaningi bangasusa ngokukhetha amaseli angaphili, okwandisa ubumsulwa benani lamaseli elifunekayo.

Ukulawulwa Kwekhwalithi Ekukhuleni Kwamaseli: I-Trypan Blue isetshenziselwa izinjongo zokulawula ikhwalithi kumalebhu okukhuliswa kwamaseli. Ngokuhlola njalo ukusebenza kwamaseli, abacwaningi bangaqapha impilo kanye nekhwalithi yamaseli akhuliswe futhi bathole noma yikuphi ukungcola okungenzeka noma ukucindezeleka kwamaseli.