I-SYBR GREEN I Nucleic Acid Gel Stain CAS:163795-75-3

I-DNA gel electrophoresis: I-SYBR Green I stain ifakwa kwi-DNA gel ngemva kwe-electrophoresis, futhi uma ivezwa ekukhanyeni kwe-UV, ibopha kuma-DNA bands, ikhiqize ukukhanya okukhanyayo. Le ndlela yokufaka imibala isiza ukubona ngeso lengqondo nokuhlaziya izingcezu ze-DNA ezihlukaniswe ngobukhulu.

I-Quantitative PCR (qPCR): I-SYBR Green I ivame ukusetshenziswa ku-qPCR ukuqapha ukukhuliswa kwe-DNA noma i-RNA sequences ngesikhathi sangempela. Idayi ibopha ku-DNA enezintambo ezimbili ekhiqizwa ngesikhathi sokukhulisa i-PCR, futhi ukwanda kokuqina kwe-fluorescence kulinganiswa kumjikelezo ngamunye wokukhulisa, okuvumela ukukhuliswa kwethempulethi ye-nucleic acid yokuqala.

I-RNA gel electrophoresis: I-SYBR Green I ingasetshenziswa futhi kwi-RNA gel electrophoresis, okuvumela ukubonakala nokuhlaziywa kwezingcezu ze-RNA. Iwusizo ekuhlolweni kobuqotho be-RNA kanye nokufunda amazinga okuvezwa kwe-RNA.

Ukulebula nokutholwa kwe-Nucleic acid: I-SYBR Green I ingasetshenziswa ukulebula ama-nucleic acid nge-fluorescence. Lokhu kungafezwa ngokufaka udayi ngesikhathi sokwenziwa kwe-nucleic acid noma ngokusebenzisa amasu okubhala ngemuva kokulebula. Ama-nucleic acid alebula angabonakala futhi atholakale kusetshenziswa izinhlelo zokuthwebula ze-fluorescent.