I-Streptavidin evela ku-Streptomyces avidinii CAS:9013-20-1

Ukuqina kokubopha: I-Streptavidin inokuqina okuphezulu kakhulu kwe-biotin, okwenza ibe ithuluzi elihle kakhulu lokubamba nokuthola ama-molecule e-biotinylated.

Ukuhlanzwa kwamaprotheni: I-Streptavidin ivame ukusetshenziswa njengethuluzi lokuhlanza ku-biotechnology. Amathegi noma ama-molecule e-biotinylated anganamatheliswa kuma-streptavidin beads, okuvumela ukuhlukaniswa nokuhlanzwa kwamaprotheni athile kusuka ezingxubeni eziyinkimbinkimbi.

Ukuhlolwa kokutholwa: I-Streptavidin ivame ukusetshenziswa ekuhlolweni kwe-immunoassays, njenge-enzyme-linked immunosorbent assays (ELISA), lapho ingabopha khona ama-antibodies okuthola ama-biotinylated noma ama-antigen. Lokhu kuvumela ukutholwa okubucayi nokuqondile kwamaprotheni noma ama-biomarker ahlukahlukene.

Ukulethwa kwemithi okuqondiwe: I-Streptavidin ingasetshenziswa ezinhlelweni zokulethwa kwemithi ngokuyihlanganisa nama-ejenti okwelapha noma ama-nanoparticles. Ama-molecule okuqondiwe e-Biotinylated angaxhunywa ku-streptavidin, okwenza kube lula ukulethwa okuqondiwe kumaseli noma ezicutshini ezithile.

Amasu ebhayoloji yama-molecular: I-Streptavidin isetshenziswa kumasu ahlukahlukene ebhayoloji yama-molecular, njengokuhlolwa kwe-nucleic acid hybridization kanye ne-polymerase chain reaction (PCR). Ama-probe noma ama-primer e-biotinylated angasetshenziswa ukuthola noma ukukhulisa ama-nucleic acid athile, bese i-streptavidin isetshenziswa ukubona ngeso lengqondo noma ukubamba imikhiqizo ye-biotinylated.

Ukuthwebula Izithombe: I-Streptavidin ivame ukusetshenziswa njengethuluzi kubuchwepheshe be-fluorescence microscopy kanye ne-imaging. Ngokuhlanganisa amadayi e-fluorescent ne-streptavidin, izinhloso ezithile zingafakwa ilebula futhi zibonwe ngokuzwela okuphezulu kanye nesisombululo.

Ukuxilongwa: Ukuhlolwa okusekelwe ku-Streptavidin kusetshenziswa kabanzi ekuxilongweni kwemitholampilo ukuze kutholakale izifo noma amagciwane. Ama-probe e-biotinylated angathola ama-nucleic acid noma ama-antigen athile, okwenza kube lula ukuxilongwa nokuqapha izimo ezahlukahlukene.