I-PUROMYCIN DIHYDROCHLORIDE CAS:58-58-2

Umphumela Ekuhlanganisweni Kwamaprotheni: I-Puromycin ifana ne-aminoacyl-tRNA engama-3' futhi ingangena endaweni ye-A yama-ribosome ngesikhathi sokuhumusha. Uma isifakiwe, ibangela ukuqedwa kokuhlanganiswa kwamaprotheni ngaphambi kwesikhathi, okuholela ekukhishweni kwama-polypeptide anqunyiwe. Lokhu kwenza i-puromycin ibe ithuluzi eliwusizo lokufunda ukuhlanganiswa kwamaprotheni kanye namazinga okuguquka kwamaseli.

Uphawu Lokukhetha: I-Puromycin ivame ukusetshenziswa njengophawu lokukhetha ekuhlolweni kobunjiniyela bezakhi zofuzo. Amaseli adluliselwe ngempumelelo noma adluliselwe ngesakhi sofuzo esifunwayo noma ukuguqulwa kwezakhi zofuzo enziwa amelana ne-puromycin ngokufaka isakhi sofuzo sokumelana ne-puromycin. Ukwengezwa kwe-puromycin ekukhuleni kwamaseli kukhetha kuphela amaseli aveza isakhi sokumelana, okuvumela ukuhlukaniswa nokusabalala kwamaseli aguqulwe izakhi zofuzo.

Ukuhlaziywa Kokusebenzisana Kwamaprotheni Namaprotheni: I-Puromycin ingahlanganiswa namathegi athile e-affinity noma ama-antibodies ukuze kufundwe ukusebenzisana kwamaprotheni namaprotheni. Ngokuhlanganisa amaprotheni asebenzisana ne-puromycin, kungenzeka ukuzinzisa nokuhlukanisa lawa ma-protein complex ukuze kuhlaziywe kabanzi.

Ucwaningo Lokuphindaphinda Kwamagciwane: I-Puromycin ingasetshenziswa ukutadisha ukuphindaphinda kwamagciwane ekukhuleni kwamaseli. Ngokufaka i-puromycin kumaseli atheleleke ngegciwane, ukwenziwa kwamaprotheni amasha egciwane kungamiswa, okuvumela abacwaningi ukuthi baphenye indima yamaprotheni athile egciwane emjikelezweni wokuphindaphinda..