I-Proteinase K ye-albhamu ye-tritirachium CAS:39450-01-6

I-Proteinase K iyi-enzyme ekhishwa ku-albhamu ye-Tritirachium fungus. Iyi-protease ye-serine ebanzi, okusho ukuthi ingahlukanisa izibopho ze-peptide ezinhlobonhlobo zamaprotheni. I-Proteinase K yaziwa ngekhono layo lokonakalisa amaprotheni ngempumelelo, okwenza ibe ithuluzi eliwusizo ezinhlelweni ezahlukahlukene.

Enye yezindlela eziyinhloko zokusebenzisa i-Proteinase K ocwaningweni lwe-molecular biology. Ivame ukusetshenziswa kumaphrothokholi okukhipha i-DNA ne-RNA ukususa amaprotheni angaphazamisa ukuhlaziywa okungezansi. I-Proteinase K iyasiza ekuhlukaniseni amangqamuzana kanye nokonakalisa amaprotheni eseli, okuvumela ukuhlukaniswa kwama-nucleic acid asezingeni eliphezulu.

I-Proteinase K isetshenziswa kabanzi ekuhlaziyweni kwamaprotheni kanye nasezifundweni zokuchaza. Ingasetshenziswa ekugayweni kwamaprotheni, okuvumela ukukhiqizwa kwezingcezu ze-peptide ezingahlaziywa ngamasu anjenge-mass spectrometry. Ngaphezu kwalokho, i-Proteinase K iwusizo ekulandeleni amaprotheni kanye nokuhlonza indawo yama-amino acid ngaphakathi kwe-polypeptide chain.

Esinye isici esibalulekile se-Proteinase K ukuhlanza izindawo ezingcolisayo. Ivame ukusetshenziswa ezindaweni zokucwaninga ze-molecular biology ukususa ama-nucleic acid, amaprotheni, kanye nezinye izinto ezingcolisayo emishinini nasezindaweni ezizungezile. I-Proteinase K izinzile kakhulu futhi iyasebenza ngaphansi kwezimo ezahlukahlukene, kufaka phakathi amazinga okushisa aphezulu kanye namazinga ahlukahlukene e-pH.