I-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide iyinhlanganisela esetshenziswa ezifundweni ezihlukahlukene ze-biochemical, ikakhulukazi ukuze kutholwe futhi kubonakale umsebenzi we-enzyme.Kuyi-substrate engenziwa i-hydrolyzed ngama-enzyme athile, okuholela ekukhululweni komkhiqizo onemibala noma we-fluorescent.
Le nhlanganisela ivame ukusetshenziswa ekuhloleni ukuthola ubukhona nomsebenzi wama-enzyme afana ne-beta-galactosidase ne-beta-glucuronidase.Lawa ma-enzyme ahlukanisa amaqembu e-acetyl ne-glucosaminide ku-substrate, okuholela ekwakhekeni kwechromophore eluhlaza okwesibhakabhaka noma eluhlaza.
Isakhiwo esiyingqayizivele se-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide sivumela ukutholakala kalula kanye nokulinganisa umsebenzi we-enzyme.Ukusetshenziswa kwayo kumasu okuhlola ahlukahlukene, okuhlanganisa i-histochemistry, i-immunohistochemistry, nokuhlola okusekelwe kumaseli, kube negalelo ekuqondeni kangcono imisebenzi yama-enzyme.