I-Nitrotetrazolium Blue chloride CAS:298-83-9

Ocwaningweni lwezinto eziphilayo kanye nezamakhemikhali, i-Nitrotetrazolium Blue chloride isebenza njengethuluzi eliwusizo lokuthola nokulinganisa ama-superoxide radicals, adlala indima ebalulekile ezinqubweni ezahlukene zomzimba kanye nezifo. Lapho isabela nge-superoxide, i-NBT yakha umkhiqizo we-blue formazan ongancibiliki, okuvumela abacwaningi ukuthi bahlole ngokubonakalayo ukuba khona nokusatshalaliswa kwe-superoxide ngaphakathi kwamasiko amaseli, izicubu, noma izinto eziphilayo eziphelele. Leli khono lenza uphenyo ngokubandakanyeka kwama-superoxide radicals ezimweni ezahlukahlukene zezinto eziphilayo, okuhlanganisa ukusayina kwamaseli, izindlela zokuzivikela zomsingathi, kanye nomonakalo we-oxidative. Ukusebenza kwamakhemikhali kwe-NBT ngama-superoxide radicals kunikeza ukuqonda ngendima yezinhlobo ze-oxygen ezisebenzayo empilweni nasezifweni, okunikeza indlela yokuhlola izimo ezihlobene nokucindezeleka kwe-oxidative njengezifo zenhliziyo, izifo ze-neurodegenerative, kanye nezimpendulo zokuvuvukala. Ngaphezu kwalokho, ukusetshenziswa kwe-NBT kufinyelela ekuhlolweni kokukhiqizwa kwe-superoxide kumamodeli okuhlola e-pathologies, okufaka isandla ekuqondeni izindlela zesifo kanye nokuthuthukiswa kokungenelela okungenzeka kokwelapha okuqondiswe ezindleleni zokucindezeleka kwe-oxidative. Ngaphezu kwalokho, ukusetshenziswa kwe-NBT akukhawulelwe ocwaningweni oluyisisekelo, njengoba kunemiphumela ekuxilongweni kwezokwelapha, ikakhulukazi ekuhlolweni komsebenzi wokuzivikela komzimba kanye nezimo zokuvuvukala. Ngokubona ngeso lengqondo ukukhiqizwa kwe-superoxide besebenzisa i-NBT, abacwaningi nodokotela bangathola ulwazi olubalulekile mayelana nemisebenzi yamangqamuzana omzimba kanye nezinqubo ze-oxidative ezingavamile ezihambisana nezifo ezahlukahlukene, ngaleyo ndlela kube lula ukuhlola kokuxilonga nokubikezela. Ekuphetheni, i-Nitrotetrazolium I-Blue chloride idlala indima ebalulekile ocwaningweni lwezinto eziphilayo kanye nezamakhemikhali, isebenza njengethuluzi eliguquguqukayo lokuthola nokubona ama-radical e-superoxide. Amandla ayo okukhiqiza i-blue formazan precipitate ehlukile lapho isabela nge-superoxide yenza kube lula uphenyo ngokucindezeleka kwe-oxidative, izimpendulo ze-immunological, kanye nezimo zesifo. Ukusetshenziswa kwe-NBT kunegalelo ekuqondeni okujulile izindima zezinhlobo ze-oxygen ezisebenzayo empilweni nasezifweni, kanye nemiphumela engaba khona ekuthuthukisweni kwamathuluzi okuxilonga kanye namasu okwelapha aqondisa izimo ezihlobene nokucindezeleka kwe-oxidative. Ucwaningo oluqhubekayo kanye nokusetshenziswa kwe-NBT cishe kuzoletha ukuqonda okwengeziwe ngokusetshenziswa kwayo futhi kufake isandla ekuthuthukisweni kokuqonda kwethu i-redox biology kanye nezinqubo ezihlobene ne-pathological.