Kunamaqhawe amaningi angadunyiswanga asizungezile, abonakala engabantu abavamile, kodwa empeleni anikela kakhulu kithi buthule. I-Proteinase K "iyiqhawe elingadunyiswanga" embonini yokuxilonga ama-molecule, yize iqhathaniswa "nelikhulu nelinamandla" embonini, i-proteinase K iphansi kakhulu kangangokuthi sesiside isikhathi singayinaki ukubaluleka kwayo. Ngokuqubuka kobhubhane olusha lomqhele, isidingo se-proteinase K sikhuphuke kakhulu, futhi ukutholakala ekhaya nakwamanye amazwe kukude kakhulu nokusetshenziswa, futhi wonke umuntu waqaphela ngokuzumayo ukuthi i-proteinase K ibaluleke kakhulu.
Iyini ukusetshenziswa kwe-proteinase K?
I-Proteinase K iyi-serine protease enomsebenzi we-enzyme ye-proteolytic futhi ingagcina umsebenzi ezindaweni eziningi ezahlukene (i-pH (4-12.5), i-buffer enosawoti omningi, izinga lokushisa eliphakeme lika-70°C, njll.). Ngaphezu kwalokho, umsebenzi we-proteinase K awuvinjelwa yi-SDS, i-urea, i-EDTA, i-guanidine hydrochloride, i-guanidine isothiocyanate, njll., futhi inani elithile lensipho lingathuthukisa nomsebenzi we-proteinase K. Ekwelashweni kwezokwelapha (igciwane kanye nokubulala amagciwane), ukudla (ukuthambisa inyama), isikhumba (ukuthambisa izinwele), ukwenza iwayini (ukucaciswa kotshwala), ukulungiswa kwe-amino acid (izimpaphe eziwohlokile), ukukhishwa kwe-nucleic acid, ukuhlanganiswa kwe-in situ, njll., i-proteinase K Kunezinhlelo zokusebenza. Isicelo esivame ukusetshenziswa kakhulu ukukhishwa kwe-nucleic acid.
I-Proteinase K ingakhiqiza zonke izinhlobo zamaprotheni kusampula, kufaka phakathi lawo ma-histone aboshwe ngokuqinile kuma-nucleic acid, ukuze ama-nucleic acid akhishwe kusampula futhi akhishwe ku-extract, okwenza kube lula isinyathelo esilandelayo sokukhipha nokuhlanzwa. Ekutholakaleni kwe-viral nucleic acid, i-proteinase K ingenye yezingxenye ezibalulekile kusisombululo sokuthola i-virus. I-Proteinase K ingaqhekeka futhi ivale i-coat protein yegciwane, ephephile ngesikhathi sokuthutha nokuthola; ngaphezu kwalokho, i-proteinase K ingaphinde yonakalise i-RNase ivimbela ukuwohloka kwe-RNA yegciwane futhi isize ekutholakaleni kwe-nucleic acid.
Udumo olusheshayo lwe-proteinase K
Kungakhathaliseki ukuthi kusemkhakheni wocwaningo lwesayensi noma emkhakheni we-IVD, ukukhishwa kwe-nucleic acid kuyindlela eyisisekelo yokuhlola, ngakho-ke i-proteinase K ibilokhu iyinto ebaluleke kakhulu. Kodwa-ke, esikhathini esidlule, i-proteinase K yayingaziwa kakhulu kunendima yayo. Ingxenye enkulu yalokhu kwakungenxa yokuthi ubudlelwano bokunikezwa kanye nokufunwa kwe-proteinase K babuzinzile kakhulu. Bambalwa abantu abangacabanga ukuthi ukunikezwa kwe-proteinase K kungaba yinkinga.
Ngokuqubuka kobhubhane olusha lomqhele, isidingo sokuhlolwa kwe-nucleic acid sikhuphuke kakhulu. Kusukela ngasekupheleni kukaJuni 2020, iShayina isiqede cishe izivivinyo ezintsha ze-crown eziyizigidi ezingama-90, futhi leli nani liyethusa kakhulu emhlabeni jikelele. Ekuhlolweni kokukhishwa kwe-nucleic acid, ukuhlushwa okusebenzayo kwe-proteinase K kungaba ngu-50-200 μg/mL. Ngokuvamile, kuthatha cishe i-100 μg ye-proteinase K ukukhipha isampula ye-nucleic acid. Ekusetshenzisweni kwangempela, ukuze kwandiswe ukusebenza kahle kokukhishwa kwe-nucleic acid, imvamisa i-Proteinase K izosetshenziswa ngenani elandayo. Ukutholwa kwe-nucleic acid ye-coronavirus entsha kulethe inani elikhulu lesidingo se-proteinase K. Ibhalansi yokuqala yokuphakelwa kanye nesidingo se-proteinase K yaphuka ngokushesha, futhi i-proteinase K yaba yinto ebalulekile yokuvimbela ubhubhane ngobusuku obubodwa.
Ubunzima ekukhiqizweni kwe-proteinase K
Nakuba ngokuthuthuka kwalesi sifo, inani elibalulekile le-proteinase K liye laziswa ngabantu, kuyadumaza ukuthi ngenxa yokuntuleka okukhulu kwe-proteinase K, zimbalwa izinkampani zasekhaya eziye zabandakanyeka ekukhiqizweni kwe-proteinase K. Lapho abantu befuna ukusungula ukukhiqizwa kwe-proteinase K Ngesikhathi senqubo yokukhiqiza, kwatholakala ukuthi i-proteinase K iyiprotheni ekhethekile kakhulu. Kuyinselele kakhulu ukwandisa amandla okukhiqiza e-proteinase K ngesikhathi esifushane.
Ukukhiqizwa okukhulu kwe-proteinase K kubhekene nobunzima obulandelayo
1. Ukubonakaliswa okuphansi
I-Proteinase K ingalimaza amaprotheni amaningi ngaphandle kokuphazamisa iseli lomnikazi wokubonakaliswa. Ngakho-ke, izinga lokubonakaliswa kwe-proteinase K ngokuvamile liphansi kakhulu. Ukuhlolwa kwezinhlelo zokubonakaliswa kanye nezinhlobo eziveza kakhulu i-proteinase K ngokuvamile kudinga umjikelezo omude.
2. Izinsalela ze-pigment nama-nucleic acid
Ukubilisa okukhulu kungenisa inani elikhulu le-pigment kanye nezinsalela ze-nucleic acid eziphethwe yi-host. Kunzima ukususa lokhu kungcola ngenqubo elula yokuhlanza, futhi ukuhlanzwa okuyinkimbinkimbi kwandisa izindleko futhi kunciphisa izinga lokubuyiselwa.
3. Ukungazinzi
I-Proteinase K ayizinzile ngokwanele, ingazikhiqiza ngokwayo, futhi kunzima ukuyigcina izinzile ku-37°C isikhathi eside ngaphandle kwesivikelo.
4. Kulula ukushesha
Uma ulungisa i-powder eyomisiwe ye-proteinase K eqandisiwe, ukuze kuqinisekiswe ukuthi okuqukethwe okuqinile kwe-proteinase K e-powder eyomisiwe eqandisiwe kukhulu, kuyadingeka ukwengeza i-ejenti yokuvikela eyomisiwe eqandisiwe ekugxilweni okuphezulu, kodwa lapho ukugxilwa kwe-proteinase K kufinyelela ku-20mg/mL nangaphezulu, kulula. Ukuhlanganiswa kwakha i-precipitate, okuletha ubunzima obukhulu ekomiseni i-proteinase K eqandisiwe enokuqukethwe okuphezulu okuqinile.
5. Ukutshalwa kwezimali okukhulu
I-Proteinase K inomsebenzi oqinile we-protease futhi ingamanzisa amanye ama-protease elabhorethri. Ngakho-ke, i-proteinase K idinga izindawo zokukhiqiza ezikhethekile, imishini, kanye nabasebenzi bocwaningo, intuthuko kanye nokukhiqiza.
Isixazululo se-XD BIOCHEM's proteinase K
I-XD BIOCHEM ineplatifomu yokuveza nokuhlanza amaprotheni avuthiwe, futhi inolwazi olucebile ekuvezeni nasekuhlanzeni amaprotheni aphinde ahlanganiswe kanye nokwenza ngcono izinqubo zokukhiqiza. Ngokwakhiwa okusheshayo kwethimba locwaningo nokuthuthukiswa, inqubo enkulu yokukhiqiza i-proteinase K inqotshiwe. Umkhiqizo wanyanga zonke wempuphu eyomisiwe eqandisiwe ungaphezu kwama-30 KG. Umkhiqizo usebenza kahle, umsebenzi okhethekile wama-enzyme amaningi, futhi awunazo izinsalela ze-cytochrome kanye ne-nucleic acid. Siyakwamukela ukuxhumana ne-XD BIOCHEM Thola iphakheji yesilingo (I-imeyili:sales@xdbiochem.comUcingo: +86 513 81163739).
Izixazululo zobuchwepheshe ze-XD BIOCHEM zifaka phakathi
Kusetshenziswa ukuhlanganiswa kwe-plasmid enamakhophi amaningi, kukhethwa izinhlobo zokuvezwa okuphezulu ezinezinga lokuvezwa elingu-8g/L, okunqoba inkinga yezinga lokuvezwa eliphansi le-proteinase K.
Ngokusungulwa kwenqubo yokuhlanza enezinyathelo eziningi, i-host cytochrome kanye nezinsalela ze-nucleic acid ze-proteinase K zasuswa ngempumelelo ngaphansi kwenani elijwayelekile.
Ngokusebenzisa ukuhlolwa okuphezulu kwezakhi zokuvikela ezivikelayo, kwakhethwa i-buffer engagcina i-proteinase K ngokuzinzile ku-37°C.
Ama-buffer okuhlola anqoba inkinga yokuthi i-proteinase K kulula ukuyihlanganisa nokuyiqeda lapho igcwele khona, futhi abeka isisekelo sokomisa i-proteinase K ngokuqukethwe okuqinile okuphezulu.
Isampula ye-XD BIOCHEM proteinase K
Ukuhlolwa kokuqina kwe-XD BIOCHEM proteinase K: ngeke kube noshintsho olukhulu emsebenzini ngemva kwezinsuku ezingu-80 ekushiseni kwegumbi
Ukuhlolwa kokuqina kwe-XD BIOCHEM proteinase K: ngeke kube noshintsho olukhulu emsebenzini ngemva kwezinsuku ezingu-80 ekushiseni kwegumbi.
Ukuqhathaniswa komphumela wokukhipha i-nucleic acid ye-XD BIOCHEM proteinase K kanye nemikhiqizo encintisanayo. Enqubweni yokukhipha i-nucleic acid, kusetshenziswa i-XD BIOCHEM kanye ne-protease K encintisanayo ngokulandelana. Ukusebenza kahle kokukhipha i-XD BIOCHEM proteinase K kuphakeme kanti inani le-Ct le-gene eqondiwe liphansi.
Isikhathi sokuthunyelwe: Disemba 31-2021