Maningi amaqhawe angaculwa abasizungezile, abonakala engabantu abajwayelekile, kodwa eqinisweni athula anikela lukhulu kithi.I-Proteinase K "iqhawe elingaziwa" embonini yokuxilonga yamangqamuzana, nakuba iqhathaniswa "nenkulu futhi enamandla" embonini, i-proteinase K inokhiye ophansi kangangokuthi kudala singakunaki ukubaluleka kwayo.Ngokuqubuka kwalolu bhubhane olusha lomqhele, isidingo se-proteinase K senyukile, futhi ukunikezwa ekhaya nakwamanye amazwe kusasele kakhulu ekusetshenzisweni, futhi wonke umuntu wavele wabona ukuthi i-proteinase K ibaluleke kakhulu.
Iyini ukusetshenziswa kwe-proteinase K?
I-Proteinase K iyi-serine protease enomsebenzi we-proteolytic enzyme futhi ingagcina umsebenzi endaweni ebanzi yezindawo (pH (4-12.5), isivimbeli esinosawoti omningi, izinga lokushisa eliphezulu lama-70°C, njll.).Ngaphezu kwalokho, umsebenzi we-proteinase K awuvinjelwa yi-SDS, i-urea, i-EDTA, i-guanidine hydrochloride, i-guanidine isothiocyanate, njll., kanye nenani elithile lokuhlanza lingabuye lithuthukise umsebenzi we-protease K. Ekwelashweni kwezokwelapha (igciwane kanye ne-microbial disinfection ), ukudla (ukuthambisa inyama), isikhumba (ukuthambisa izinwele), ukwenziwa kwewayini (ukucaciswa kotshwala), ukulungiswa kwe-amino acid (izimpaphe ezonakele), ukukhishwa kwe-nucleic acid, in situ hybridization, njll., proteinase K Kukhona izicelo.Isicelo esisetshenziswa kakhulu yi-nucleic acid extraction.
I-Proteinase K ingakwazi ukwenza i-enzymolyze zonke izinhlobo zamaprotheni kusampula, okuhlanganisa nalawo ma-histones aboshwe ngokuqinile kuma-nucleic acid, ukuze ama-nucleic acid akhishwe kusampula futhi akhishwe ku-extract, kube lula isinyathelo esilandelayo sokukhipha nokuhlanzwa.Ekutholweni kwe-viral nucleic acid, i-proteinase K ingenye yezingxenye ezibalulekile kusixazululo sesampula segciwane.I-proteinase K ingaqhekeka futhi ingasebenzi iphrotheni yejazi yegciwane, ephephe kakhudlwana phakathi nesigaba sokuthutha nesokuthola;ngaphezu kwalokho, i-proteinase K ingakwazi futhi ukululaza i-RNase ivimbela ukuwohloka kwe-RNA yegciwane futhi yenza kube lula ukutholwa kwe-nucleic acid.
Udumo lwasebusuku lweproteinase K
Kungakhathaliseki ukuthi kumkhakha wocwaningo lwesayensi noma emkhakheni we-IVD, ukukhishwa kwe-nucleic acid kuwukuhlola okuyisisekelo, ngakho i-proteinase K ibilokhu iwukuphila okubaluleke kakhulu.Kodwa-ke, esikhathini esidlule, i-proteinase K yayingaziwa kakhulu kunendima yayo.Ingxenye enkulu yalokhu kwakungenxa yokuthi ubudlelwano bokunikezwa nokufunwa kwe-proteinase K babuzinzile kakhulu.Bambalwa abantu abangacabanga ukuthi ukuhlinzekwa kwe-proteinase K kungaba yinkinga.
Ngokuqubuka kobhubhane olusha lomqhele, isidingo sokuhlolwa kwe-nucleic acid sesikhulile.Kusukela ngasekupheleni kukaJuni 2020, iChina isiqede ukuhlolwa komqhele okusha okucishe kube yizigidi ezingama-90, futhi lesi sibalo sesabisa kakhulu emhlabeni jikelele.Ezivivinyweni zokukhishwa kwe-nucleic acid, ukugxila kokusebenza kwe-proteinase K cishe ku-50-200 μg/mL.Ngokuvamile, kuthatha cishe i-100 μg ye-proteinase K ukukhipha isampula ye-nucleic acid.Ekusetshenzisweni kwangempela, ukuze kwandiswe ukusebenza kahle kokukhipha i-nucleic acid, imvamisa iProteinase K izosetshenziswa enanini elikhuphukile.Ukutholwa kwe-Nucleic acid ye-coronavirus entsha kulethe inani elikhulu lesidingo se-proteinase K.Ibhalansi yokuqala yokunikezwa kanye nesidingo ye-proteinase K yaphuka ngokushesha, futhi i-proteinase K yaba into ebalulekile yokuvimbela ubhubhane ngobusuku obubodwa.
Ubunzima ekukhiqizeni i-proteinase K
Nakuba ngokuthuthukiswa kwalolu bhubhane, inani elibalulekile le-proteinase K liye laziswa abantu, kungamahloni ukuthi ngenxa yokhiye ophansi owedlulele we-proteinase K, zimbalwa izinkampani zasekhaya eziye zahileleka ekukhiqizeni i-proteinase K. Lapho abantu bafuna ukusungula ukukhiqizwa kwe-proteinase K Phakathi nenqubo yokukhiqiza, kwatholakala ukuthi i-proteinase K iyiphrotheni ekhetheke kakhulu.Kuyinselele enkulu kakhulu ukwandisa umthamo wokukhiqiza we-proteinase K ngesikhathi esifushane.
Ukukhiqizwa kwe-proteinase K enkulu kubhekene nobunzima obulandelayo
1. Inkulumo ephansi
I-proteinase K angonakalisi ngokungaqondile amaphrotheni amaningi futhi ibangele ubuthi obuyingozi kuseli lomsingathi.Ngakho-ke, izinga lenkulumo ye-proteinase K ngokuvamile liphansi kakhulu.Ukuhlolwa kwezinhlelo zokukhuluma kanye nezinhlobo eziveza kakhulu i-proteinase K ngokuvamile kudinga umjikelezo omude.
2. Izinsalela zezingulube nama-nucleic acid
Ukuvutshelwa kwesilinganiso esikhulu kwethula inani elikhulu le-pigment kanye nezinsalela ze-nucleic acid.Kunzima ukususa lokhu kungcola ngenqubo elula yokuhlanza, futhi ukuhlanzwa okuyinkimbinkimbi kwandisa izindleko futhi kunciphisa izinga lokubuyisela.
3. Ukungazinzi
I-proteinase K ayizinzile ngokwanele, iyakwazi ukuzinza i-enzymolyze, futhi kunzima ukuyigcina iqinile ku-37 ° C isikhathi eside ngaphandle kwe-ejenti evikelayo.
4. Kulula ukudlidliza
Lapho ulungiselela impushana eqandisiwe ye-proteinase K, ukuze uqinisekise ukuthi okuqukethwe okuqinile kwe-proteinase K kumpushana omisiwe kukhulu, kuyadingeka ukwengeza i-ejenti yokuvikela efriziwe endaweni ephakeme, kodwa uma ukuhlushwa kwe-proteinase K kufinyelela ku-20mg/mL nangaphezulu, kulula Ukuhlanganisa kwenza imvula, okuletha ubunzima obukhulu ekumisweni yiqhwa kwe-proteinase K enokuqukethwe okuqinile okuphezulu.
5. Utshalomali olukhulu
I-proteinase K inomsebenzi oqinile we-protease futhi ingakhiqiza amanye ama-protease emanzini elabhorethri.Ngakho-ke, i-proteinase K idinga izindawo ezikhethekile zokukhiqiza, okokusebenza, kanye nabasebenzi bocwaningo nokuthuthukiswa nokukhiqizwa.
Isixazululo se-XD BIOCHEM sikaproteinase K
I-XD BIOCHEM ine-protein evuthiwe yokukhuluma kanye neplathifomu yokuhlanza, futhi inokuhlangenwe nakho okucebile ekukhulumeni nasekuhlanzeni amaprotheni ahlanganisiwe kanye nokwenza kahle kwezinqubo zokukhiqiza.Ngokwakhiwa okusheshayo kwethimba locwaningo nentuthuko, inqubo enkulu yokukhiqiza ye-proteinase K isinqotshiwe.Ukukhishwa kwanyanga zonke kwempushana eqandisiwe kungaphezu kuka-30 KG.Umkhiqizo unokusebenza okuzinzile, umsebenzi othize we-enzyme ephezulu, futhi azikho izinsalela ze-cytochrome ne-nucleic acid.Siyakwamukela ukuthintana ne-XD BIOCHEM Thola iphakheji yesilingo (I-imeyili:sales@xdbiochem.comUcingo: +86 513 81163739).
Izixazululo zobuchwepheshe ze-XD BIOCHEM zihlanganisa
Kusetshenziswa ukuhlanganiswa kwe-plasmid enamakhophi amaningi, izinhlobo zokuveza okukhulu ezineleveli yokusho engu-8g/L ziyakhethwa, okunqoba inkinga yezinga lokuvezwa eliphansi le-proteinase K.
Ngokusungulwa kwenqubo yokuhlanza enezinyathelo eziningi, izinsalela ze-cytochrome ne-nucleic acid ze-proteinase K zikhishwe ngempumelelo ngaphansi kwevelu evamile.
Ngokuhlolwa komphumela ophezulu wokwakheka kwebhafa yokuvikela, isigcinalwazi esingagcina ngokuzinzile i-proteinase K ku-37°C sikhethiwe.
Izinqamuli zokuhlola zinqoba inkinga yokuthi i-proteinase K kulula ukuyihlanganisa kanye nemvula ekugxiliseni okuphezulu, futhi ibeka isisekelo sokumisa-iqhwa kokuqukethwe okuphezulu kwe-proteinase K.
Isampula ye-XD BIOCHEM proteinase K
Ukuhlolwa kokuzinza kwe-XD BIOCHEM proteinase K: ngeke kube khona ushintsho olubalulekile emsebenzini ngemva kwama-80 d ekamelweni lokushisa
Ukuhlolwa kokuzinza kwe-XD BIOCHEM proteinase K: ngeke kube khona ushintsho olubalulekile emsebenzini ngemva kwama-80 d ekamelweni lokushisa.
Ukuqhathaniswa komphumela wokukhishwa kwe-nucleic acid we-XD BIOCHEM proteinase K kanye nemikhiqizo encintisanayo.Enqubweni yokukhipha i-nucleic acid, i-XD BIOCHEM kanye ne-proteinase K yokuncintisana kusetshenziswa ngokulandelana.Ukusebenza kahle kokukhipha kwe-XD BIOCHEM proteinase K kuphezulu futhi inani le-Ct lofuzo oluqondiwe liphansi.
Isikhathi sokuthumela: Dec-31-2021