I-N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine usawoti wesodium I-CAS:102062-46-4

Usawoti we-sodium i-N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine uyinhlanganisela yamakhemikhali evame ukusetshenziswa ezindaweni ezahlukene zelebhu. Uyisithako esincibilika emanzini se-tetramethylbenzidine (TMB) esiqukethe iqembu le-sulfopropyl.

Le nhlanganisela ivame ukusetshenziswa njenge-substrate ekuhlolweni kwe-colorimetric ukuthola ukuthi kukhona ama-enzyme afana nama-peroxidases noma i-horseradish peroxidase (HRP). Uma la ma-enzyme ekhona, angavuselela ukusabela nge-N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine sodium salt, okuholela ekwakhiweni komkhiqizo onombala. Ukuqina kombala kuhambisana ngqo nomsebenzi we-enzyme, okuvumela ukulinganiswa kwe-enzyme noma ukutholakala kwama-substrate athile.

Ukuncibilika kwamanzi kwe-N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine sodium salt kwenza kube lula ukusetshenziswa ezixazululweni zamanzi futhi kuhambisana nezinhlelo ezahlukene zokuhlola. Iqembu layo le-sulfopropyl lithuthukisa ukuncibilika kanye nokuqina kwenhlanganisela, okuvumela ukutholwa okusebenzayo nokuthembekile ekuhlolweni kwe-enzyme.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-Immunohistochemistry (IHC): I-STMB isetshenziswa njenge-substrate ye-chromogenic ku-IHC ukuze kubonwe ubukhona bama-antigen athile kumasampula ezicubu. Umbala ophumayo uvumela ukuhlonza kanye nokutholakala kwamaprotheni aqondiwe.

I-ELISA: I-STMB isetshenziswa kabanzi ku-ELISA kanye ne-enzyme immunoassays (EIA) ukuthola nokulinganisa ama-target analytes afana namaprotheni, ama-hormone, nama-antibodies. Ukusabela kwe-colorimetric okuqhutshwa yi-STMB kunikeza isignali elinganiswayo yokuba khona kanye nokuhlushwa kwe-analyte.

Ukuqothula kwe-Western: I-STMB isetshenziswa njengesisekelo sama-antibodies esibili ahlanganiswe ne-HRP kukuqothula kwe-western ukuze kubonwe ubukhona bamaprotheni aqondiwe. Umbala ophumelayo uvumela ukutholakala okuqondile kwamaprotheni athakazelisayo kulwelwesi.

Ukuhlolwa komsebenzi we-enzyme: I-STMB ingasetshenziswa ukukala umsebenzi we-peroxidase wama-enzyme ahlukene. Ngokuqapha ukukhiqizwa kombala, abacwaningi banganquma umsebenzi we-enzyme futhi bahlole ukusebenza kahle kokusabela kwe-enzyme.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C19H25N2NaO3S
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	102062-46-4
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.