I-MTT (i-Thiazolyl blue tetrazolium bromide) I-CAS:298-93-1

I-MTT, noma i-Thiazolyl blue tetrazolium bromide, iyi-reagent evame ukusetshenziswa ku-biology yamaseli kanye ne-biochemistry ukuhlola ukusebenza kwamaseli kanye nokwanda kwawo. Uma i-MTT ingezwa kumaseli aphilayo, incishiswa ama-enzyme e-mitochondrial ukuze kwakheke umkhiqizo we-purple formazan. Lokhu kuguqulwa kuvame ukusetshenziswa njengesibonakaliso somsebenzi we-metabolic ngaphakathi kwamaseli. Ukuhlolwa kwe-MTT kusetshenziswa kabanzi ekutholakaleni kwezidakamizwa, ukuhlolwa kwe-cytotoxicity, kanye nokutadisha izindlela zokubonisa amaseli. Ukusetshenziswa kwayo kuvumela abacwaningi ukuthi balinganise imiphumela yamakhemikhali ahlukahlukene empilweni kanye nomsebenzi wamaseli, okwenza kube ithuluzi eliwusizo ekuqondeni ukuziphatha kwamaseli kanye nezimpendulo ekwelashweni.