I-Hygromycin B CAS:31282-04-9

Uma usebenzisa i-hygromycin B ekuhlolweni kwe-cell culture noma i-molecular biology, kubalulekile ukuphatha i-compound ngokucophelela futhi ulandele izinqubo zokuphepha ezifanele. Ukuze ulungiselele isisombululo se-hygromycin B sokukhetha amaseli, ncibilikisa ufulawa ku-solvent efanele njengamanzi noma i-ethanol ukuze ufinyelele ukuhlushwa okufiselekayo okunconyelwe ukusetshenziswa kwakho okukhethekile. Faka isisombululo se-hygromycin B ku-cell culture media ekuhlushweni okufanele ukuze ubulale amaseli angamelani, uvumele amaseli aguquliwe noma angamelani kuphela ukuthi asinde futhi ande. Qapha ukuhlushwa kwamaseli njalo ukuqinisekisa ukukhetha okufanele nokukhula kwamaseli afunwayo. Ngezicelo ze-molecular biology, faka i-hygromycin B ku-growth media yamaseli aguquliwe ukuze ugcine ingcindezi yokukhetha kanye nokubonakaliswa okuzinzile kwama-resistance gene. Yenza ukuhlolwa okuvamile kanye nokuhlolwa ukuqinisekisa ukuzinza nokusebenza kahle kokukhethwa kwe-hygromycin B ekugcineni ukuguqulwa kwezakhi zofuzo okufunwayo. Njalo bheka ulwazi lomkhiqizo kanye neziqondiso zobuchwepheshe ezinikezwe umenzi ukuze uthole imiyalelo ethile yokuphatha, ukulungiselela, kanye nokusetshenziswa kwe-hygromycin B ekuhlolweni kwakho. Khumbula ukugqoka imishini yokuzivikela efanele futhi usebenze endaweni yelabhoratri ekhethiwe lapho usebenza nale antibiotic ukuqinisekisa ukuphepha nokuvimbela ukungcola.