HEPES CAS:7365-45-9 Intengo Yomkhiqizi

Ukubhafa kwe-pH: I-HEPES isetshenziswa kabanzi njenge-ejenti yokubhafa ukuze kugcinwe i-pH ezinzile kumidiya yesiko lamaseli kanye nokuhlolwa kwebhayoloji.Isebenza ngempumelelo ebangeni le-pH ye-physiological engu-6.8 kuya ku-8.2, iyenze ifaneleke ukugcina izimo ezilungile zezinqubo zebhayoloji.

Isiko lamaseli: I-HEPES ivamise ukungezwa kumidiya ye-cell culture ukuze kunzinziswe izinga le-pH, ihlinzeke ngendawo efanelekile yokukhula kwamaseli nokugcina ukusebenza kwamaseli.Kuyasiza ukuvimbela ukushintshashintsha kwe-pH okungase kube nomthelela omubi ekuziphatheni kwamaseli nemiphumela yokuhlola.

Ukuhlolwa kwama-enzyme: I-HEPES ivamise ukusetshenziswa njengesivimbeli ekuphenduleni kwe-enzymatic ngenxa yekhono layo lokugcina i-pH ethile.Kuyasiza ekugcineni umsebenzi olungile we-enzymatic nokuzinza, ngaleyo ndlela kuthuthukisa ukunemba nokuthembeka kokuhlolwa kwe-enzyme.

Izifundo zamaphrotheni: I-HEPES iqashwe ekuhloleni okuhlukahlukene okuhlobene namaprotheni, okuhlanganisa ukuhlanzwa kwamaprotheni, ukuqina kwamaprotheni, nokuhlaziywa kwesakhiwo samaprotheni.Kuyasiza ukuzinzisa i-pH futhi kugcinwe ukugoqa okufanele kwamaprotheni kanye nokuzinza phakathi nalokhu kuhlolwa.

I-Electrophoresis: I-HEPES ithola ukusetshenziswa kumasu e-gel electrophoresis, njenge-SDS-PAGE kanye ne-agarose gel electrophoresis.Isetshenziswa njenge-ejenti yokubhafa kusigcinalwazi esisebenzayo, ihlinzeka ngendawo ye-pH ezinzile yokuhlukaniswa nokuhlaziywa kwama-biomolecules.

Ukwakhiwa kwemithi: I-HEPES isetshenziswa ekwakhiweni kwemikhiqizo yezemithi neye-biotechnological.Kuyasiza ukugcina ukuzinza nobuqotho bezithako ezisebenzayo, ukuqinisekisa ukusebenza kahle kwazo kanye nempilo yeshalofu.