I-Geneticin disulfate (i-G418 sulfate) I-CAS:108321-42-2

Ezindaweni zelebhu, i-geneticin disulfate (i-G418 sulfate) ivame ukusetshenziswa ekuhlolweni kwamasiko eseli ukukhetha nokugcina imigqa yamaseli equkethe ama-vector okubonakaliswa anezakhi zofuzo zokumelana, njenge-neomycin phosphotransferase. Lawa maseli angasinda lapho kukhona i-geneticin disulfate ngenxa yokubonakaliswa kwezakhi zofuzo zokumelana, okuvumela abacwaningi ukuthi bahlukanise futhi bafunde imiphakathi yamaseli aguquliwe ngokwezakhi zofuzo. Abacwaningi basebenzisa i-geneticin disulfate ezicelweni ezahlukahlukene, okuhlanganisa ukukhiqizwa kwemigqa yamaseli okuzinzile, amasu okuhlela izakhi zofuzo njenge-CRISPR-Cas9, kanye nokukhiqizwa kwamaprotheni ahlanganisiwe. Ngokufaka i-geneticin disulfate endaweni yokukhulisa, imiphakathi ethile yamaseli ingacebiswa noma ihlukaniswe ngokusekelwe ekhonweni layo lokusinda ekukhethweni kwama-antibiotic. Ukwenza ngcono ukuhlushwa kanye nobude bokwelashwa kwe-geneticin disulfate kubalulekile ukuze kufezwe ingcindezi ekhethiwe ngenkathi kunciphisa ubuthi be-cytotoxicity kanye nemiphumela engeyona eyomgomo kumaseli angaguquliwe. Ukuqapha ngokucophelela ukuphila kwamaseli, izici zokukhula, kanye namaphethini okubonakaliswa kwezakhi zofuzo kusiza ukuqinisekisa imiphumela yokuhlolwa ephumelelayo lapho kusetshenziswa i-geneticin disulfate ezifundweni zamasiko eseli. Abacwaningi kumele balandele izinqubo ezimisiwe zokusetshenziswa kwe-geneticin disulfate, okuhlanganisa nezinqubo ezifanele zokulinganisa, ukuphatha, kanye nokulahla, ukuze kugcinwe amazinga okuphepha kwelabhorethri futhi kukhuthazwe imiphumela yesayensi enembile. Ukubambisana phakathi kososayensi, abasebenzi belabhorethri, kanye neziphathimandla ezilawulayo kusekela ukusetshenziswa okunesibopho nokusebenzayo kwe-geneticin disulfate ocwaningweni lwebhayoloji yama-molecular, okuthuthukisa ukuqonda kwethu izinqubo zamaseli kanye namasu okuphatha izakhi zofuzo.