I-Disodium 4-nitrophenylphosphate CAS:4264-83-9

Umphumela oyinhloko kanye nokusetshenziswa kwe-disodium 4-nitrophenylphosphate ukusetshenziswa kwayo njenge-substrate ye-chromogenic yokuhlola i-enzyme ye-alkaline phosphatase. Uma le nhlanganisela ihlanganiswa ne-alkaline phosphatase, i-enzyme ihlukanisa iqembu le-phosphate ku-disodium 4-nitrophenylphosphate, okuholela ekwakhekeni kwe-4-nitrophenol.

I-4-nitrophenol ekhiqizwayo iyinhlanganisela enombala ophuzi engatholakala nge-spectrophotometric ku-405 nm. Lokhu kushintsha kombala kusetshenziselwa ukulinganisa ngobuningi umsebenzi we-alkaline phosphatase kumasampula, njenge-biological fluids noma i-cell lysates.

Ukusetshenziswa kwe-disodium 4-nitrophenylphosphate ekuhlolweni kwe-enzyme kunikeza indlela elula yokulinganisa umsebenzi we-alkaline phosphatase. Le enzyme ihilelekile ezinqubweni ezahlukahlukene zebhayoloji futhi ivame ukusetshenziswa njengophawu lokuhlukanisa izicubu, ukusebenza kwesibindi, kanye nokuguqulwa kwamathambo.

Ngaphezu kwalokho, i-disodium 4-nitrophenylphosphate ingasetshenziswa njengesisekelo sezinye i-phosphatase, njenge-acid phosphatase noma i-protein phosphatase, kuye ngokuthi kusetshenziswe kanjani ukuhlola kanye nomgomo wocwaningo.