I-Concanavalin A CAS:11028-71-0 Intengo Yomkhiqizi

I-Concanavalin A (Con A) iyi-lectin, okuwuhlobo lweprotheni engakwazi ukubopha ngqo kuma-molecule athile kashukela. Itholakala embewini yesitshalo se-jackbean (i-Canavalia ensiformis).

I-Con A inamandla aphezulu okunamathelana kwezinsalela zikashukela ze-mannose kanye ne-glucose futhi ingabopha kuma-glycoprotein nama-glycolipid aqukethe lawa shukela. Yakha ama-complexes aguqukayo nalawa ma-molecule ngokusebenzisana kwama-carbohydrate nama-protein.

Ngenxa yekhono layo lokubopha ushukela, i-Con A ivame ukusetshenziswa ocwaningweni lwezinto eziphilayo kanye nokuxilonga. Isetshenziswe kabanzi njengethuluzi lokufunda ama-glycoprotein, ushukela ongaphezulu kweseli, kanye nokusebenzisana okusekelwe kuma-carbohydrate. Ingasetshenziswa ukuhlukanisa nokuhlanza ama-glycoprotein nge-affinity chromatography, kanye nokuthola nokulinganisa izingxenye ezithile zikashukela ezindaweni zeseli noma ezingxubeni eziyinkimbinkimbi.

I-Con A nayo inezakhiwo zokulawula amasosha omzimba. Ingakhuthaza ukusebenza nokwanda kwamaseli athile omzimba, njenge-T lymphocytes, futhi ihlolwe ngezinhlelo zayo zokwelapha ezingaba khona ekwelashweni kwamasosha omzimba kanye namasu okugoma.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukuhlanganiswa Kwamaseli: Omunye wemiphumela ephawulekayo ye-Con A yikhono layo lokubangela ukuhlanganiswa kwamaseli. Lesi sici sibe usizo ekutadisheni ukunamathela kwamaseli kanye nokusebenzisana kobuso bamaseli.

Ukuhlukaniswa kwe-Glycoprotein: I-Con A ibopha ngqo kuma-glycoprotein aqukethe i-mannose yokugcina kanye nezinsalela ze-glucose. Lesi sici sisetshenziswe ukuhlukanisa nokuhlanza ama-glycoprotein ezingxubeni eziyinkimbinkimbi, njenge-biological fluids noma i-cell lysates, ngokusebenzisa i-Con A affinity chromatography.

Ukutholwa kwamaGlycoprotein: I-Con A ingasetshenziswa ukuthola nokulinganisa ama-glycoprotein kumasampula. Ngokusebenzisa i-Con A ehlanganiswe namathegi e-fluorescent noma e-enzymatic, kungenzeka ukubona ngeso lengqondo noma ukukala inani lama-glycoprotein akhona kusampula ngokusebenzisa amasu e-fluorescence microscopy noma amasu e-enzyme-linked immunosorbent assay (ELISA), ngokulandelana.

Ukulebula Okungaphezulu Kweseli: Ngenxa yokuthi ihambisana noshukela ongaphezulu kweseli, i-Con A ingasetshenziswa ukulebula izinhlobo ezithile zamaseli noma ukutadisha amaphethini e-glycosylation ezindaweni zamaseli. I-Con A ehlanganiswe nama-fluorophores noma amanye amathegi ivumela ukubonakala nokuhlaziywa kwamaseli ngamasu anjenge-flow cytometry noma i-fluorescence microscopy.

Ukuguqulwa Kwamasosha omzimba: I-Con A inezakhiwo zokuguqula amasosha omzimba futhi ingathinta ukusebenza kwamaseli omzimba. Ingakhuthaza ukusebenza nokwanda kwama-T lymphocyte, okuholela ekukhiqizweni kwama-cytokine nezinye izimpendulo zamasosha omzimba. Le mfanelo ihlolwe ekwelashweni kwamasosha omzimba kanye nokuthuthukiswa kwemithi yokugoma, lapho i-Con A isetshenziswe khona ukuthuthukisa izimpendulo zamasosha omzimba noma ukulawula ukubekezelelana kwamasosha omzimba.

Ithuluzi Lokucwaninga: I-Con A iyithuluzi elisetshenziswa kabanzi emikhakheni ehlukahlukene yocwaningo lwezinto eziphilayo. Isetshenziswe ukutadisha ukusebenzisana kwama-carbohydrate nama-protein, ukunamathela kwamaseli, amaphethini e-glycosylation, kanye nezimpendulo zomzimba. Amandla ayo okubopha kushukela othize ayenza ibe usizo ekutadisheni nasekuphatheni ama-glycoprotein nama-glycolipid.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C15H24
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	11028-71-0
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.