I-Avidin CAS:1405-69-2 Intengo Yomkhiqizi

Ukuhlanzwa Kwamaprotheni: I-Avidin ingavinjelwa ngokusekelwa okuqinile, njenge-chromatography resin noma ama-magnetic beads, futhi isetshenziswe ukubamba amaprotheni ahlanganisiwe noma ama-nucleic acid. Le ndlela yaziwa ngokuthi i-avidin affinity chromatography, futhi ivumela ukuhlanzwa kwama-molecule athile ngendlela ecacile kakhulu.

Ama-Probes Ane-Biotinyled: I-Avidin ivame ukuhlanganiswa nama-enzyme, ama-fluorophores, noma amanye amalebula futhi isetshenziswa njenge-reagent yokuthola ezivivinyweni ezahlukahlukene. Ama-probes ane-Biotinyled, njenge-antibodies noma ama-nucleic acid probes, angatholakala ngokubopha kuma-molecule okuthola axhumene ne-avidin. Le ndlela ivame ukusetshenziswa ku-ELISA, i-immunohistochemistry, kanye ne-nucleic acid hybridization assays.

Ukulethwa Kwemithi Okuqondiwe: I-Avidin ingasetshenziswa njenge-molecule yokuthwala ukuletha imithi noma izinto zokwelapha ngqo kumaseli noma izicubu eziveza ama-receptor e-biotin. Ngokuhlanganisa i-biotin nemithi noma ama-nanoparticles nokuzixhumanisa ne-avidin, izinhlelo zokulethwa kwemithi eziqondiwe zingadalwa.

Amasu Ebhayoloji Yamamolekyuli: Izinhlelo ezisekelwe ku-Avidin zisetshenziswa kabanzi ocwaningweni lwebhayoloji yamamolekyuli. Isibonelo, i-streptavidin, enobuhlobo obufanayo ne-biotin njenge-avidin, isetshenziswa kumasu afana nokulandelana kwe-DNA, i-PCR, kanye ne-in situ hybridization, lapho ihlanganiswa khona nama-probe noma ama-primer ahlanganisiwe.

Ukuthwebula Izithombe Nokuxilonga: I-Avidin ingafakwa ilebula ngama-radioisotopes, amadayi e-fluorescent, noma ama-nanoparticles, okwenza kube ithuluzi eliwusizo kumasu okuthwebula izithombe njenge-positron emission tomography (PET), i-fluorescence microscopy, kanye ne-magnetic resonance imaging (MRI). Ngaphezu kwalokho, izinhlelo ezisekelwe ku-avidin zisetshenziswa ekuhlolweni kokuxilonga, njengokuhlolwa kwe-immunoassays, ukuthola ukuba khona kwama-biomarker athile.