I-AMPPD CAS: 122341-56-4 Intengo Yomkhiqizi

i-AMPPD, okufushane ngoba I-3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane, iyi-substrate ye-chemiluminescent evame ukusetshenziswa ekusetshenzisweni kwezinto eziphilayo kanye nokuhlolwa kwe-immuno. Isetshenziswa kabanzi ocwaningweni lwe-molecular biology, ekuxilongeni, kanye nasesayensini yokuphila.

I-AMPPD yenzelwe ngqo ukusetshenziswa kanye nama-enzyme e-alkaline phosphatase conjugates. Uma ivezwa ku-alkaline phosphatase, i-AMPPD ibhekana nokusabela okusheshayo nokuphumelelayo kokuqhekeka kwe-enzyme, okuholela ekukhishweni kokukhanya.

Ukukhanya okukhishwe yi-AMPPD kutholwa kusetshenziswa izinhlelo zokuthwebula izithombe ezibucayi noma ama-luminometer, okuvumela ukulinganiswa kanye nokubonakala kwama-molecule noma ama-analyte aqondiwe. Le ndlela yokuthola esekelwe ku-chemiluminescence inikeza ukuzwela okuphezulu, umsindo ophansi wangemuva, kanye nobubanzi obuhlukahlukene.

Ngenxa yokuqina kwayo kanye nesibonakaliso esihlala isikhathi eside, i-AMPPD ifaneleka kahle ezinhlelweni zokusebenza ezifana ne-Western blotting, i-ELISA (i-enzyme-linked immunosorbent assay), i-DNA hybridization, kanye ne-in situ hybridization. Ivumela abacwaningi ukuthola nokuhlaziya amaprotheni, ama-nucleic acid, ama-antibodies, kanye namanye ama-biomolecule.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-AMPPD ivame ukusetshenziswa kanye ne-enzyme alkaline phosphatase. Uma ivezwa ku-alkaline phosphatase, i-AMPPD ibhekana nokusabela kokuqhekeka okudala isignali yokukhanya. Lokhu kukhishwa kokukhanya kungatholakala futhi kukalwe kusetshenziswa amathuluzi akhethekile, okuvumela ukubonakala kanye nokulinganisa ama-biomolecule aqondiwe.

Ukusetshenziswa kwe-AMPPD kusakazeke kabanzi ezindleleni ezahlukene, okuhlanganisa i-Western blotting, i-ELISA (i-enzyme-linked immunosorbent assay), i-DNA ne-RNA hybridization, kanye ne-in situ hybridization. Lezi zindlela zisetshenziswa ukutadisha amaprotheni, ama-nucleic acid, ama-antibodies, kanye namanye ama-molecule ezinto eziphilayo.

I-AMPPD inikeza izinzuzo eziningana, okuhlanganisa ukuzwela okuphezulu, umsindo ophansi wangemuva, kanye nobubanzi obuhlukahlukene obuguquguqukayo. Isignali yayo ezinzile nehlala isikhathi eside iyenza ifaneleke kokubili ukuhlaziywa kwekhwalithi kanye nokwezibalo.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C16H28N6O10P2
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	122341-56-4
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.