4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS:3459-18-5 Isithombe Esifakiwe

I-4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS:3459-18-5

I-4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside iyinhlanganisela yamakhemikhali evame ukusetshenziswa ocwaningweni lwe-biochemical kanye ne-molecular biology. Iyi-substrate eyaziwa kakhulu ngokuthi i-pNAG, esetshenziswa kakhulu ekuhlolweni kwe-enzyme ukuthola nokulinganisa umsebenzi wama-enzyme afaka i-hydrolyze izibopho ze-β-D-glucoside.

I-pNAG ayinambala futhi ayikhanyi ukukhanya, kodwa uma i-hydrolysis iqhutshwa ama-enzyme athile, ikhiqiza umkhiqizo ophuzi, i-p-nitrophenol. Lesi sici senza i-pNAG ibe usizo ekutholeni nasekulinganiseni umsebenzi wama-enzyme afana nama-β-glucosidases, akhuthaza ukuqhekeka kwezibopho ze-β-D-glucoside.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-Enzyme Substrate: i-pNAG isetshenziswa kabanzi njenge-substrate ethile yama-enzyme ahlukahlukene ahilelekile ekugayweni kwezibopho ze-β-D-glucoside. Lapho la ma-enzyme ehlukanisa i-molecule ye-pNAG, ikhipha i-p-nitrophenol. Lokhu kuvumela abacwaningi ukuthi balinganise futhi balinganise umsebenzi we-enzymatic.

Ukuhlolwa Komsebenzi We-Enzyme: Ukuhlanzwa kwe-pNAG ngama-enzyme athile kungatholakala futhi kulinganiswe nge-spectrophotometric. Lokhu kwenza i-pNAG ifaneleke ekuhlolweni komsebenzi we-enzyme, lapho inani le-p-nitrophenol elikhiqizwayo lilingana ngqo nomsebenzi we-enzymatic.

Ukuhlolwa Komthamo Ophezulu: I-pNAG ivame ukusetshenziswa ekuhlolweni komthamo ophezulu ukuhlonza nokuchaza ama-enzyme inhibitors noma ama-activator. Ngokuhlola umphumela wamakhemikhali ahlukene emsebenzini we-enzyme, abacwaningi bangahlonza abantu abangaba khona abazosebenzisa izidakamizwa noma abaguquli bomsebenzi we-enzyme.

Izifundo Zokuvezwa Kwezakhi Zofuzo: i-pNAG isetshenziswa futhi ocwaningweni lwebhayoloji yama-molecule ukutadisha ukubonakaliswa kwezakhi zofuzo kanye nokulawulwa kwazo. Ngokulinganisa umsebenzi we-enzymatic wama-enzyme athile kusetshenziswa i-pNAG njenge-substrate, abacwaningi bangaphenya ngomphumela wokubonakaliswa kwezakhi zofuzo emsebenzini kanye nomsebenzi we-enzyme.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C14H18N2O8
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	3459-18-5
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.