3,3′,5,5′-Tetramethylbenzidine sulfate CAS:54827-18-8

I-ELISA: Isetshenziswa kabanzi njengesisekelo se-horseradish peroxidase (HRP) ekuhlolweni kwe-ELISA. Uma kukhona i-molecule eqondiwe, njenge-antigen noma i-antibody, i-enzyme i-HRP ivuselela i-oxidation ye-3,3', 5,5'-Tetramethylbenzidine sulfate, okuholela ekwakhekeni komkhiqizo oluhlaza okwesibhakabhaka. Ukuqina kombala kuhambisana ngqo nokuhlushwa kwe-molecule eqondiwe, okuvumela ukuhlaziywa kwenani.

Ukuhlolwa komsebenzi we-enzyme: 3,3', 5,5'-Tetramethylbenzidine sulfate ingasetshenziswa ukukala umsebenzi we-enzyme. Ngokufaka isampula ye-enzyme nge-substrate, ukusabela kwe-enzyme kungaqashwa ngokushintsha kombala. Umsebenzi we-enzyme ophezulu uholela kumbala oluhlaza okwesibhakabhaka oqinile, okwenza kube lula ukunqunywa kwe-enzyme kinetics kanye nezifundo zokuvinjelwa.

I-Immunohistochemistry: Ingasetshenziswa ezinqubweni zokufaka umbala ku-immunohistochemistry (IHC). Isetshenziswa njenge-chromogen yama-antibodies ayinhloko noma awesibili abhalwe nge-horseradish peroxidase, okuholela ekwakhekeni kwediphozithi eluhlaza okwesibhakabhaka ebonakalayo endaweni yokusebenzisana kwe-antigen-antibody.

Ucwaningo lwe-biochemical: 3,3', 5,5'-Tetramethylbenzidine sulfate ithola ukusetshenziswa ezifundweni ezahlukene zocwaningo lwe-biochemical, ikakhulukazi ekutholeni nasekulinganisweni kwemisebenzi ye-enzyme, ukusebenzisana kwamaprotheni namaprotheni, kanye nokusebenzisana kwamaprotheni ne-DNA.