3,3′-Diaminobenzidine CAS:91-95-2

Umphumela we-3,3'-Diaminobenzidine (DAB) ukukhiqiza umbala onsundu uma isabela kuma-enzyme e-peroxidase. Lokhu kushintsha kombala kuvumela ukubonakala kwama-antigen aqondiwe noma ama-biomolecule anentshisekelo ekuhlolweni kombala we-immunohistochemistry (IHC) kanye ne-immunocytochemistry (ICC). I-DAB ivame ukusetshenziswa ocwaningweni lwezokwelapha kanye nezicelo zokuxilonga ukuthola nokuthola ama-antigen athile noma amaprotheni ngaphakathi kwamasampula ebhayoloji. Ngokufaka i-DAB njenge-substrate kanye nokusabela okulandelayo ngama-enzyme e-peroxidase, kwakheka i-precipitate ensundu endaweni yama-antigen aqondiwe. Ukusetshenziswa kombala we-DAB kuwusizo kakhulu ekutadisheni izifo nezimo ezahlukahlukene. Kwenza abacwaningi kanye nososayensi bezifo bakwazi ukubona nokuhlaziya ukusatshalaliswa, ubuningi, kanye nokwenziwa kwendawo kwama-biomolecule athile ezicutshini noma emaseli. Lolu lwazi lusiza ekuqondeni izindlela zesifo, ukutadisha izinqubo zamaseli, kanye nokuhlonza izinhloso zokuxilonga noma zokwelapha ezingaba khona. Ngaphezu kwalokho, ukufaka i-DAB kungasetshenziswa ezindleleni ezahlukene zama-molecule ukuphenya ukusebenzisana kwamaprotheni, ukuhlonza amaphethini okuvezwa kwamaprotheni, ukunquma ukuba khona kwezimpawu ezithile, kanye nokuhlola ukusebenza kahle kokungenelela kokwelapha. Ngenxa yokudaya kwayo okuthembekile nokuqinile, kanye nokuhambisana kwayo nezinhlelo ezahlukene zokuthola kanye namasu e-microscopy, i-DAB isibe yi-substrate ye-chromogenic esetshenziswa kabanzi ekuhlolweni kwe-immunohistochemistry kanye ne-immunocytochemistry.