3,3′-Diaminobenzidine CAS:91-95-2

I-3,3′-Diaminobenzidine (DAB) iyinhlanganisela yamakhemikhali evame ukusetshenziswa ku-biochemistry kanye ne-histology yokufaka ama-protein, ama-nucleic acid, namanye ama-macromolecule. Uma i-oxidized, yakha i-precipitate enombala onsundu ebonakala kalula ngaphansi kwe-microscope. Ukufaka ama-DAB kuvame ukusetshenziswa ukuthola ubukhona kanye nendawo yama-molecule athile, njenge-antigens noma ama-enzyme, kumaseli nasezicutshini. Kuyindlela ethandwayo yokukhetha amasu e-immunohistochemistry kanye ne-immunocytochemistry ngenxa yokuzwela kwayo okuphezulu kanye nokuqina. Ukufaka ama-DAB kunganikeza ukuqonda okubalulekile ngezakhiwo zamaseli kanye nokusebenzisana kwama-molecule, kusize ocwaningweni nasekuxilongweni emikhakheni efana nemithi, i-biology, kanye ne-pathology.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-Immunohistochemistry: Ukufaka i-DAB staining kusetshenziswa kakhulu ku-IHC ukuthola nokuthola amaprotheni noma ama-antigen athile ezingxenyeni zezicubu. Le ndlela isiza abacwaningi kanye nodokotela bezifo ukuthi bafunde ngesimo seseli, bathole izingxenye zeseli, bahlole amaphethini okuvezwa kwamaprotheni, futhi bahlole izinguquko ezihlobene nesifo. Ukufaka i-DAB staining kungasetshenziswa emikhakheni efana nocwaningo lomdlavuza, i-neuroscience, i-pathology, kanye ne-biology yentuthuko.

I-Immunocytochemistry: Ukupenda i-DAB kusetshenziswa futhi ku-ICC ukubona ngeso lengqondo ama-molecule aqondiwe kumaseli akhuliswe kahle. Le ndlela ifana ne-IHC, evumela abacwaningi ukuthi bahlole ukuvezwa kwamaprotheni, indawo, kanye nokuguquguquka kwamaseli ku-vitro.

Ukuxutshwa kwe-In situ: Ukuxutshwa kwe-DAB kungasetshenziswa ekutholeni ukulandelana kwe-DNA noma kwe-RNA ethile kusetshenziswa amasu okuxutshwa kwe-In situ. Ngokuhlanganisa ukuxutshwa kwe-DAB nama-probe aqondisa ukulandelana kwe-nucleic acid okuhambisanayo, abacwaningi bangabona ngeso lengqondo indawo kanye nokubonakaliswa kwezakhi zofuzo ngaphakathi kwamaseli noma izicubu.

I-electron microscopy: Ukufaka i-DAB kungasetshenziswa ku-electron microscopy ukuze kufakwe ilebula ezakhiweni ezithile noma ama-molecule athakazelisayo. Uma kuhlanganiswa nokufaka i-heavy metal ngemuva kokufaka i-dain, ukufaka i-DAB kungathuthukisa umehluko kanye nokubonakala kwezingxenye zamaseli ezifundweni ze-ultrastructural.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C12H14N4
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	91-95-2
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.