3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

Ukutholwa komsebenzi we-beta-galactosidase: I-ONPG ivamise ukusetshenziselwa ukunquma ubukhona nomsebenzi we-beta-galactosidase kumasampula ebhayoloji ahlukahlukene, njengamasiko ebhaktheriya noma ama-lysates eseli.Ukukhiqizwa kwe-o-nitrophenol, enombala ophuzi, kungalinganiswa kalula kusetshenziswa i-spectrophotometer.

Izifundo ze-Gene expression: I-ONPG ivamise ukusetshenziswa ocwaningweni lwebhayoloji yamangqamuzana ukutadisha isisho sofuzo.Ngokuhlanganisa umgqugquzeli wofuzo lwentshisekelo ne-beta-galactosidase efaka ikhodi yofuzo, abacwaningi bangakala umsebenzi walo mgqugquzeli ngokungeza i-ONPG futhi balinganisele umphumela wokukhiqizwa kwe-o-nitrophenol.Le ndlela, eyaziwa ngokuthi i-beta-galactosidase reporter assay, inikeza ulwazi mayelana nomsebenzi wokuloba wofuzo.

Ukuhlonza amagciwane: Amanye amagciwane akhiqiza i-beta-galactosidase, kanti amanye awayenzi.I-ONPG ingasetshenziswa ngokuhlanganiswa nezinye izivivinyo ze-biochemical ukukhomba izinhlobo zebhaktheriya ngokusekelwe emandleni azo okwenza i-ONPG i-hydrolyze.Le ndlela ivame ukusetshenziswa kumalabhorethri okuxilonga emitholampilo kanye ne-microbiology.

Ukuhlolwa kwama-enzyme inhibitors noma ama-activators: I-ONPG ingasetshenziselwa ukuhlola izinhlanganisela ezishintsha umsebenzi we-beta-galactosidase.Ngokulinganisa umsebenzi we-enzyme phambi kwezinhlanganisela ezahlukene, abacwaningi bangakwazi ukuhlonza ama-inhibitor angaba khona noma ama-activator angaphinde aphenywe ngamakhono azo okwelapha.