2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

I-2-Nitrophenyl-beta-D-glucopyranoside iyinhlanganisela yamakhemikhali ehlanganisa i-molecule ye-glucopyranoside exhunywe eqenjini le-nitrophenyl.Ngokuvamile isetshenziswa njenge-substrate ekuhlolweni kwe-enzymatic ukuthola kanye nokulinganisa umsebenzi wama-enzyme njenge-beta-glucosidase.Iqembu le-nitrophenyl lingaqhekeka yi-enzyme, okuholela ekukhululweni komkhiqizo onombala ophuzi ongalinganiswa nge-spectrophotometrically.Le nhlanganisela iwusizo ikakhulukazi ekutadisheni i-enzyme kinetics kanye nokuhlolwa okuphezulu kwama-enzyme inhibitors noma ama-activator.Iphinde isetshenziswe ocwaningweni lwamakhemikhali ezinto eziphilayo ukuze kuphenywe nge-carbohydrate metabolism nanjenge-glycosidic-linkage-specific substrate.

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Imininingwane Yomkhiqizo

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Isicelo kanye Nomphumela

I-Enzyme substrate: I-ONPG ivamise ukusetshenziswa njenge-substrate ye-beta-galactosidase, i-enzyme eyenza i-ONPG i-hydrolyze ukuze ikhiqize inhlanganisela enombala ophuzi (o-nitrophenol) engabonwa kalula nge-spectrophotometrically.Lokhu kusabela kwe-enzymatic kungasetshenziswa ukukala umsebenzi we-beta-galactosidase, okwenza i-ONPG ibe ithuluzi elibalulekile ezifundweni ze-enzymology.

Ukuhlolwa kwebhayoloji yamangqamuzana: I-ONPG isetshenziswa njengengxenye ekuhlolweni okuhlukahlukene kwebhayoloji yamangqamuzana, ikakhulukazi ekuhlolweni kofuzo lwentatheli ye-beta-galactosidase.Kulezi zivivinyo, i-substrate esekelwe ku-ONPG isetshenziselwa ukukala umsebenzi wofuzo lwentatheli, oluvamise ukulawulwa ukulandelana komgqugquzeli othile wentshisekelo.Umsebenzi we-beta-galactosidase, oboniswa ukushintsha kombala okukhiqizwa ku-ONPG hydrolysis, unikeza ulwazi olubalulekile mayelana nomsebenzi womgqugquzeli.

Ukuhlaziywa kwesisho sofuzo: I-ONPG nayo isetshenziswa ekuhlaziyeni isisho sofuzo.Ngokuxhumanisa ukulandelana komgqugquzeli wentshisekelo kuhlobo lwe-beta-galactosidase, abacwaningi bangakala umsebenzi we-beta-galactosidase basebenzisa i-ONPG njenge-substrate.Izinga lomsebenzi we-beta-galactosidase libonisa amandla nomsebenzi womgqugquzeli, okuvumela ukuhlolwa kwamaleveli esisho sofuzo.

Izinhlelo zokuxilonga: I-ONPG ingasetshenziswa ezinhlelweni zokuxilonga, ikakhulukazi ekutholeni amagciwane e-pathogenic.Amagciwane ahlukahlukene, njenge-Escherichia coli nezinhlobo ezithile ze-Shigella ne-Salmonella, akhiqiza i-beta-galactosidase engahlukanisa i-ONPG.Lokhu kusabela kwe-hydrolysis kukhiqiza ukuguqulwa kombala okubonakalayo, okungasetshenziswa ukunquma ukuba khona kwalawa magciwane kumasampula omtholampilo.

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Ukupakishwa komkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	I-C12H15NO8
Isivivinyo	99%
Ukubukeka	Impushana ephuzi ekhanyayo
Inombolo ye-CAS.	2816-24-2
Ukupakisha	Ezincane nezinkulu
I-Shelf Life	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	ISO.