I-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS:24404-53-3

I-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ivame ukusetshenziswa njenge-substrate ye-enzyme i-β-galactosidase. Uma i-β-galactosidase isebenza kuyo, i-compound iyashiswa, okuholela ekukhiqizweni kwe-p-nitrophenol noma i-o-nitrophenol, enombala ophuzi ongatholakala kalula. Lokhu kuyenza ibe usizo njenge-substrate yokuhlolwa okukala umsebenzi we-β-galactosidase.

Ukusetshenziswa okuvamile kwe-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside kusezihlolweni zezakhi zofuzo zentatheli. Kulezi zivivinyo, izakhi zofuzo ezithakaselwayo zihlanganiswa nezakhi zofuzo zentatheli, njengezakhi zofuzo ze-beta-galactosidase. Umsebenzi wezakhi zofuzo zentatheli ube usuqashwa ngokulinganisa umsebenzi we-β-galactosidase kusetshenziswa i-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside njenge-substrate. Lokhu kuvumela abacwaningi ukuthi batadishe ukubonakaliswa kwezakhi zofuzo kanye nokulawulwa kwazo.

I-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ingasetshenziswa futhi ukuhlola ama-inhibitors noma ama-activators e-β-galactosidase. Ngokuhlola ama-compounds ahlukene noma ama-candidate emithi, abacwaningi bangabona ama-molecule alawula umsebenzi wale enzyme, angaba nezicelo zokwelapha ezifweni noma ezimweni ezithile.

Ngaphezu kwalokho, le nhlanganisela ingasetshenziswa ekuhlanzweni kwamaprotheni. I-β-galactosidase ivame ukuhlanganiswa namaprotheni athakazelisayo ukuze kube lula ukuwathola nokuwahlanza. I-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ingasetshenziswa njengesisekelo sokuhlola nokuhlanza la maprotheni okuhlanganiswa ngokuthola umsebenzi we-β-galactosidase.