I-2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

I-2-Nitrophenyl-beta-D-glucopyranoside iyinhlanganisela yamakhemikhali equkethe i-molecule ye-glucopyranoside exhunywe eqenjini le-nitrophenyl. Ivame ukusetshenziswa njenge-substrate ekuhlolweni kwe-enzyme ukuthola nokulinganisa umsebenzi wama-enzyme afana ne-beta-glucosidase. Iqembu le-nitrophenyl lingahlukaniswa yi-enzyme, okuholela ekukhishweni komkhiqizo ophuzi ongalinganiswa nge-spectrophotometric. Le nhlanganisela iwusizo kakhulu ekutadisheni i-kinetics ye-enzyme kanye nokuhlolwa okuphezulu kwe-enzyme inhibitors noma ama-activator. Iphinde isetshenziswe ocwaningweni lwe-biochemical ukuze kuphenywe nge-metabolism ye-carbohydrate kanye ne-substrate ethize ye-glycosidic-linkage.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-substrate ye-enzyme: I-ONPG ivame ukusetshenziswa njenge-substrate ye-beta-galactosidase, i-enzyme e-hydrolyze i-ONPG ukuze ikhiqize i-compound ephuzi (i-o-nitrophenol) engatholakala kalula nge-spectrophotometric. Lokhu kusabela kwe-enzymatic kungasetshenziswa ukukala umsebenzi we-beta-galactosidase, okwenza i-ONPG ibe ithuluzi eliwusizo ezifundweni ze-enzymology.

Ukuhlolwa kwebhayoloji yama-molecular: I-ONPG isetshenziswa njengesisekelo ezivivinyweni ezahlukene zebhayoloji yama-molecular, ikakhulukazi ezivivinyweni ze-beta-galactosidase reporter gene. Kulezi zivivinyo, isisekelo esisekelwe ku-ONPG sisetshenziselwa ukukala umsebenzi we-reporter gene, ovame ukulawulwa yi-promoter sequence ethile yesithakazelo. Umsebenzi we-beta-galactosidase, oboniswa ushintsho lombala olukhiqizwa yi-ONPG hydrolysis, unikeza ulwazi olubalulekile mayelana nomsebenzi we-promoter.

Ukuhlaziywa kokubonakaliswa kwezakhi zofuzo: I-ONPG isetshenziswa futhi ekuhlaziyweni kokubonakaliswa kwezakhi zofuzo. Ngokuxhumanisa ukulandelana kwentshisekelo yephromotha nezakhi zofuzo ze-beta-galactosidase, abacwaningi bangalinganisa umsebenzi we-beta-galactosidase besebenzisa i-ONPG njengesisekelo. Izinga lomsebenzi we-beta-galactosidase libonisa amandla nomsebenzi wephromotha, okuvumela ukuhlolwa kwamazinga okubonakaliswa kwezakhi zofuzo.

Izicelo zokuxilonga: I-ONPG ingasetshenziswa ekuxilongweni, ikakhulukazi ekutholeni amagciwane abangela izifo. Amagciwane ahlukahlukene, njenge-Escherichia coli kanye nezinhlobo ezithile ze-Shigella kanye ne-Salmonella, akhiqiza i-beta-galactosidase engahlukanisa i-ONPG. Lokhu kusabela kwe-hydrolysis kukhiqiza ushintsho lombala olubonakalayo, olungasetshenziswa ukunquma ukuba khona kwala magciwane kumasampula omtholampilo.

Isampula Yomkhiqizo

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C12H15NO8
Ukuhlolwa	99%
Ukubukeka	Impuphu ephuzi ekhanyayo
Inombolo ye-CAS	2816-24-2
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.